HAFC from Creative Bioarray are isolated from annulus fibrosus of human intervertibral disc. HAFC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HAFC are characterized by immunofluorescent method with antibodies to fibronectin and vimentin. HAFC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAFC are guaranteed to further expand for 15 population doublings in the condition provided by Creative Bioarray.