Okadaic Acid?DSP?ELISA Test Kit

Business Type
by email /anna@reagen.us (Get Latest Price)

Quick Details

Brand Name: REAGEN
Model Number: RNA97008
Place of Origin: USA
MOQ: 2kits
Supply Type: 500kits/month
Standard: 96T
Packaging: color box
Okadaic Acid(DSP)ELISA Test Kit: high sensitivity

Product Detail

REAGEN™Okadaic Acid (DSP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Okadaic Acid (DSP) in mussels and water samples. The unique features of the kit are: ?High recovery (80-105%), rapid (10-30 minutes), and cost-effective extraction methods ?High sensitivity (0.3 ng/g or ppb) and low detection limit (30 ppb for mussel samples and 6 ppb for water samples). ?High reproducibility. ?A quick ELISA assay(less than 1.5 hours regardless of number of samples). Procedure Overview The method is based on a competitive colorimetric ELISA assay. The second antibody against Anti-Okadaic Acid Antibody was coated in the plate wells. During the analysis, sample is added along with the okadaic acid-horseradish peroxidase conjugate (Okadaic Acid-HRP Conjugate) and Anti- Okadaic Acid Antibody. If the okadaic acid residue is present in the sample, it will compete with Okadaic Acid-HRP Conjugate for the Anti-Okadaic Acid Antibody, thereby preventing the Okadaic Acid-HRP Conjugate from binding to the Anti-Okadaic Acid Antibody. The Anti-Okadaic Acid Antibody is then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the okadaic acid residue concentration in the sample. Sensitivity Sample Type Detection Limit (ng/g or ppb) Mussels 30 Water 6

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